Report on the Demonstration of the Use of Zebrafish Embryo HTS Platform for Rapid Hazard Assessment of Commercial Pristine Slurry

  • Authors:
    Olivia Osborne (UCLA), Wen Jiang (UCLA), Andre Nel (UCLA)
    Publication ID:
    P089993
    Publication Type:
    Deliverable Report
    Received Date:
    20-Dec-2016
    Last Edit Date:
    13-Jan-2017
    Research:
    425.050 (University of California/Los Angeles)

Research Report Highlight

Report summarizes the experimental results of pristine slurry with use of zebrafish high throughput to perform hazard assessment and dosimetry. Furthermore, it includes the characterization data of commercially available pristine slurry.

Abstract

This report summarizes the experimental results of pristine slurry with use of zebrafish high throughput (HTS) to perform hazard assessment and dosimetry. Furthermore, it includes the characterization data of commercially available pristine slurry. The hazard potential of the pristine slurry was firstly determined based on the percentage of embryo hatching, rate of survival, and morphological abnormalities in response to pristine slurry. This demonstrated that pristine slurry didn’t exert effects on zebrafish embryo hatching or survival up to 200 ppm. Larval screening demonstrated pristine had adverse impact on survival/growth as well as biopersistence which we surmise is a result of ultrastructural damage observed in the gastrointestinal tract (GIT) from the pristine slurry complex mixture which include oxidizers, heavy metals and biocides. This report demonstrates an assessable platform for the environmental hazard potential of commercially available pristine CMP slurries on fish species as well as help in de-convoluting the complexity of industrial CMP slurries which is the research we are currently undertaking.

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